ENVIRONMENTAL BEST PRACTICES CONFERENCE call for papers

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Please note a new phone/fax number:
phone: 48 89 523 4119
fax: 48 89 523 4119
e-mail: ebp@uwm.edu.pl

IUPAC Sponsored Conference

027772

THE FIRST INTERNATIONAL ENVIRONMENTAL BEST PRACTICES CONFERENCE
07-10 August 2006, OLSZTYN, POLAND

ABSTRACT SUBMISSION

General information

all abstracts must be written in English

please, limit your abstract to approximately 200 words

all abstracts must be submitted either:

via web site - ON-LINE SUBMISSION
via E-mail (in Word format) to address: brzuzan@uwm.edu.pl

Deadline for abstract submission: 31^st of May, 2006
all abstracts will be evaluated by the Steering Committee members,a book of abstracts will be distributed at the conference.

ON-LINE SUBMISSION

TITLE

AUTHOR NAMES

for each author enter the full name, surname in capitals

separate authors by commas

follow each author's name by a consecutive number for their affiliation

insert an asterisk (*) or underline the name of the author who will present the paper
ex: Agnieszka CYDZIK-KWIATKOWSKA^*1, Slawomir CIESIELSKI², Irena WOJNOWSKA-BARYŁA¹

AFFILIATIONS

in the line after the "authors" enter the affiliation number between parenthesis followed by the corresponding affiliation:

name of the Institution/Organisation/Company
name of the Unit/Department/Section
name of the City, State, Province, Country

ex: (1) University of Warmia and Mazury, Department of Environmental Biotechnology, 10-957 Olsztyn, Poland

MAIN TEXT

Please check in which of the following categories your paper should be placed
Environmental Remediation
Wastewater Treatment
Alternative Energy Sources
Environmental Stewardship
Solid Waste Management
Other (please consider proposing a theme):

Would you like your abstract to be considered for

oral presentation
yes no

poster presentation yes no

Your e-mail

EXAMPLE OF THE ABSTRACT

Bacterial amoA gene expression in activated sludge during SBR cycle

Agnieszka CYDZIK-KWIATKOWSKA^*1, Slawomir CIESIELSKI², Irena WOJNOWSKA-BARYŁA¹

(1) Department of Environmental Biotechnology, (2) Department of Evolutionary Genetics; University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-957 Olsztyn-Kortowo, Poland
Transcription levels of amoA mRNA over an operating cycle of SBR reactor was analyzed using reverse transcriptase PCR (RT-PCR). Relationship between amoA mRNA expression in activated sludge aggregates and changes in nitrogen compounds concentration was examined. First expression of amoA gene was detected after two hours of aeration and then gradually increased. Lack of amoA expression at the beginning of the aeration phase was caused by a high concentration of organic carbon, which stimulated growth of heterotrophic bacteria rather than development of nitrifying microorganisms. Amount of amoA transcripts gradually increased with the increase in the activity of ammonia-oxidizing bacteria. We concluded that amoA gene expression correlated well with the increase in nitrate concentration. However, amoA gene expression did not immediately respond to the decrease in ammonium oxidation activity. The RT-PCR-based approach allowed to interrelate transcription level of amoA mRNA with the conditions of SBR reactor environment, providing indirect information on the activity of ammonia oxidizing bacteria during the wastewater treatment processes.

^* Corresponding author; phone and fax +48 89 5233754, agnieszka.cydzik@uwm.edu.pl